| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | Briefly, cells were aseptically isolated from femurs and tibia of C57BL/6 mice and cultured in complete DC media comprised of RPMI-1640 (Gibco) supplemented with 10% fetal bovine serum (FBS, Corning), 1% penicillin/ streptomycin (Gibco), 55 µM β-mercaptoethanol (Gibco), 20 ng/ml ACS Paragon Plus Environment Page 21 of 31 Nano Letters 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 granulocyte–macrophage colony-stimulating factor (GM-CSF, Genscript) at 37°C with 5% CO2. | Get A Quote |
Innate immune cells recognize and respond to pathogen-associated molecular patterns. In particular, polysaccharides found in the microbial cell wall are potent activators of dendritic cells (DCs). Here, we report a new class of nanocapsules, termed sugar-capsules, entirely composed of polysaccharides derived from the microbial cell wall. We show that sugar-capsules with a flexible polysaccharide shell and a hollow core efficiently drain to lymph nodes and activate DCs. In particular, sugar-capsules composed of mannan (Mann-capsule) carrying mRNA (mRNA) promote strong DC activation, mRNA translation, and antigen presentation on DCs. Mann-capsules elicit robust antigen-specific CD4+ and CD8α+ T-cell responses wi... More
