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Proteins, Expression, Isolation and Analysis | All primers were designed, and the constructed plasmids were sequenced in two directions by GenScript Bio Co.... Purification of the Recombinant BpLipase Enzyme and SDS–PAGE Analysis The N-terminally attached His-tag lipase was purified using an immobilized metal ion affinity chromatography column (GenScript, China). | Get A Quote |
To investigate whether Bombyx mori Linnaeus (Lepidoptera: Bombycidae) intestinal microorganism play a role in the host defence system against viral pathogens, a lipase gene from the silkworm intestinal bacterium Bacillus pumilus SW41 was characterized, and antiviral activity of its protein against B. mori nucleopolyhedrovirus (BmNPV) was tested. The lipase gene has an open-reading frame of 648 bp, which encodes a 215-amino-acid enzyme with a 34-amino-acid signal peptide. The recombinant lipase (without signal peptide) was expressed and purified by using an Escherichia coli BL21 (DE3) expression system. The total enzyme activity of this recombinant lipase reached 277.40 U/mg at the optimum temperature of 25°C a... More