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Immobilization of actively thromboresistant assemblies on sterile blood-contacting surfaces

Adv Healthc Mater. 2015;

Qu Z, Krishnamurthy V, Haller CA, Dorr BM, Marzec UM, Hurst S, Hinds MT, Hanson SR, Liu DR, Chaikof EL.

Products/Services Used	Details	Operation
Recombinant Proteins	… Expression and purification of recombinant TM LPETG and sortases, as well as solution phase sortase transpeptidation reactions are detailed in the Supporting Information. Gly 5 ‐biotin was obtained from Genscript, Piscataway and used without further purification …	Get A Quote

摘要

Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.,Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.