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In Vitro Detection of Cellular Adjuvant Properties of Human Invariant Natural Killer T Cells

Methods Mol Biol. 2019;

Zhang R, Kitayama S, Liu T, Ueda N, , Tokumitsu Y, Mashima H, Ohdan H, Kaneko S, Uemura Y, .

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摘要

Invariant natural killer T (iNKT) cells are a subset of T lymphocytes that play a crucial role in the tumor surveillance. The activation of iNKT cells by their specific ligand α-galactosylceramide (α-GalCer) induces the activation of dendritic cells (DCs) via reciprocal interaction, which results in the generation of cellular immunity against cancer. Here we describe a method to detect DC-mediated cellular adjuvant properties of human iNKT cells in vitro.