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Catalog Antibody | Dcp1a was detected using mouse monoclonal anti-Dcp1a antibody (ab57654; Abcam, Cambridge, MA), and TTP was detected using a rabbit polyclonal antibody against amino acids 19 to 33 of human TTP (GenScript, Piscataway, NJ) | Get A Quote |
Transforming growth factor β (TGF-β) is a potent growth regulator and tumor suppressor in normal intestinal epithelium. Likewise, epithelial cell growth is controlled by rapid decay of growth-related mRNAs mediated through 3′ untranslated region (UTR) AU-rich element (ARE) motifs. We demonstrate that treatment of nontransformed intestinal epithelial cells with TGF-β inhibited ARE-mRNA expression. This effect of TGF-β was promoted through increased assembly of cytoplasmic RNA processing (P) bodies where ARE-mRNA localization was observed. P-body formation was dependent on TGF-β/Smad signaling, as Smad3 deletion abrogated P-body formation. In concert with increased P-body formation, TGF-β induced expressi... More