| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | The cells were treated with increasing doses of CQ as described in (a), after which cell viability was measured by propidium iodide incorporation analysis by flow cytometry and trypan blue staining analysis by hemocytometer. c Tc17 cells were generated as described in (a) with CD8+ T cells that were magnetically isolated from WT mice. d Flow cytometry analysis of T-bet and STAT1 from samples described in (a). e WT mice were s.c. immunized with 200 µg of MOG35–55peptide (GenScript) emulsified in complete Freund’s adjuvant containing 5 mg/mL M. | Get A Quote |
Chloroquine (CQ) is a weak base that was originally used to treat malaria infection.1 However, recent findings have pinpointed the modulatory effect of CQ in models of chronic inflammation and viral infections. The antiviral effect of CQ and its derivative hydroxychloroquine has attracted great attention due to the recent Sars-CoV-2 virus outbreak.2,3 As an immunomodulatory agent, CQ reduces the severity of experimental autoimmune encephalomyelitis (EAE), likely by inducing regulatory T cells and tolerogenic dendritic cells (DCs).4,5 EAE and multiple sclerosis (MS) are demyelinating disorders of the CNS in which interleukin (IL)-17-producing helper T (Th17) cells play a major role in disease severity. To dat... More
