| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | … Samples were heated at 98 °C for 8 min, separated on a 10% Tris-glycine acrylamide gel (BioRad), transferred to PVDF membrane and incubated with either anti-V5 or anti-HA primary antibody (Genscript) followed by a HRP conjugated anti-mouse secondary antibody … | Get A Quote |
Little is known about Epstein Barr Virus (EBV) proteins that participate in viral DNA cleavage and packaging. Genes encoding potential terminase subunit and portal protein homologs include BGRF1/BDRF1, BALF3, BFRF1A and BBRF1 respectively. EBV mutants with deletions in one or more of these genes were impaired for DNA packaging (Pavlova et al., 2013). In the current study, BBRF1 oligomers were purified from recombinant baculovirus infected insect cell extracts. Transmission electron microscopy revealed that purified EBV portals retained features typically found in other portals including a central channel with clip, stem and wing/crown domains. Although compounds have been identified that target DNA encapsidatio... More
