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Proteins, Expression, Isolation and Analysis | … The protein fraction (LtxA) was concentrated using Amicon Ultra (Merck, Kenilworth, New Jersey, USA) The amount of LPS in 1 μg of LtxA was 4 ng, as detected by the LPS-detection kit (GenScript, Piscataway Township, NJ, USA) Page 8 This article is protected by copyright … | Get A Quote |
Aggregatibacter actinomycetemcomitans is considered to be associated with periodontitis. Leukotoxin (LtxA), which destroys leukocytes in humans, is one of this bacterium's major virulence factors. Amounts of neutrophil elastase (NE), which is normally localized in the cytoplasm of neutrophils, are reportedly increased in the saliva of patients with periodontitis. However, the mechanism by which NE is released from human neutrophils and the role of NE in periodontitis is unclear. In the present study, it was hypothesized that LtxA induces NE release from human neutrophils, which subsequently causes the breakdown of periodontal tissues. LtxA-treatment did not induce significant cytotoxicity against human gingival... More