资源 » 参考数据库 » 客户发表文献

至今，GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次，处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果，再次证明GenScript 有能力帮助业内科学家Make research easy.

Engineered Functional Recovery of Microbial Rhodopsin Without Retinal-Binding Lysine.

Photochem Photobiol. 2019;

Yamauchi Y, Konno M,, Yamada D, Yura K,,, Inoue K,,, Béjà O, Kandori H,.

Products/Services Used	Details	Operation
Codon Optimization	… Mutagenesis and protein expression The synthesized genes of ABL3, ABL4 and SAMEA51 codon- optimized to E coli (GenScript, USA) were incorporated into pET21a(+) vector within N-terminal NdeI and C-terminal XhoI restriction sites (Merck KGaA, Germany) …	Get A Quote

摘要

Definition of rhodopsin is the retinal-binding membrane protein with the Schiff base linkage at a lysine on the 7th transmembrane helix. However, ~ 600 microbial rhodopsins lack retinal-binding lysine at the corresponding position (Rh-noK) among ~ 5500 known microbial rhodopsins, suggesting that Rh-noK has each functional role without chromophore. Here, we report successful functional recovery of Rh-noK. Two Rh-noKs from bacteria were heterologously expressed in Escherichia coli, which exhibited no color. When retinal-binding lysine was introduced, one of them gained visible color. Additional mutation of the Schiff base counterion further gained proton-pumping activity. Successful engineered functional recove... More