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Codon Optimization | … Evaluation of the appropriate codon for E coli expression was done in GenScript rare codon analysis (https://wwwgenscriptcom/tools/rare-codon-analysis) using codon adaptation index (CAI) with an ideal value of 08-1, GC content (ideal at 30–70%), codon frequency … | Get A Quote |
Nattokinase is an enzyme produced by Bacillus subtilis subsp. natto that contains strong fibrinolytic activity. It has potential to treat cardiovascular diseases. In silico analysis revealed that nattokinase is considered as an antigen, thus hindering its application for injectable therapeutic protein. Various web servers were used to predict B-cell epitopes of nattokinase both continuously and discontinuously to determine which amino acid residues had been responsible for the immunogenicity. With the exclusion of the predicted conserved amino acids, four amino acids such as S18, Q19, T242, and Q245 were allowed for mutation. Substitution mutation was done to lower the immunogenicity of native nattokinase. Thro... More