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Proteins, Expression, Isolation and Analysis | … Estimation of caspase-8 and -9 activities Caspase-8 and -9 enzyme activities were measured colorimetrically using commercially available kits (GenScript, Piscataway, NJ, USA) according to the man- ufacturer's instructions. Page 3. Suramin in liver cancer 1050 … | Get A Quote |
To investigate the cytotoxic activity of suramin against HepG2 human HCC cell line.,HepG2 cells were treated with 15, 30 and 45 μM suramin. HepG2 cell proliferation was measured by MTT and lactate dehydrogenase (LDH) assays. Heparan sulfate proteoglycans (HSPGs), glucuronic acid and glucosamine levels were measured. Moreover, apoptosis was assessed by measuring caspase-8 and caspase-9 activities. The effect of suramin on HepG2 cells was compared with the same doses of a standard drug, cisplatin.,Suramin blocked heparanase leading to dose-dependent increase in HSPGs and reduction in glucuronic acid and glucosamine levels. Suramin reduced HepG2 cells survival and showed cell cytotoxicity in a dose-dependent mann... More