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Codon Optimization | … The cloning and purification for the human CKs were made as previously reported [22]. The DNA sequence encod- ing amino acid residues 79-440 of PfCK was made syntheti- cally and codon optimised by GenScript to be expressed in E. coli … | Get A Quote |
Choline kinase (CK) is a homodimeric enzyme that catalyses the transfer of the ATP γ-phosphate to choline, generating phosphocholine and ADP in the presence of magnesium. Several isoforms of CK are present in humans but only the HsCKα has been associated with cancer and validated as a drug target to treat this disease. As a consequence a large number of compounds based on Hemicholinium (HC-3) have been described. Two compounds, previously reported to inhibit the human enzyme, have recently been shown to inhibit P. falciparum CK (PfCK) and therefore their potential applications might be anticipated to other pathogens. Herein, using molecular dynamic simulations, we have firstly observed that the ATP and the ch... More