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Proteins, Expression, Isolation and Analysis | … proteins were separated by SDS PAGE, transferred to poly(vinyl idene difluoride) membrane, and visualized using anti VKOR (Genscript, China) and … cysteines probably has a role in maintaining structure [2]. VKORs from S. lycopersicum and A. thaliana can cat alyze disulfide … | Get A Quote |
Homologs of vitamin K epoxide reductase (VKOR) exist widely in plants. However, only VKOR of Arabidopsis thaliana has been the subject of many studies to date. In the present study, the coding region of a VKOR from Solanum lycopersicum (JF951971 in GenBank) was cloned; it contained a membrane domain (VKOR domain) and an additional soluble thioredoxin-like (Trx-like) domain. Bioinformatic analysis showed that the first 47 amino acids in the N-terminus should act as a transit peptide targeting the protein to the chloroplast. Western blot demonstrated that the protein is localized in thylakoid membrane with the Trx-like domain facing the lumen. Modeling of three-dimensional structure showed that SlVKOR has a simil... More