| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … lacks the N-terminal mitochondrial targeting sequence (MTS) and hydrophobic sequences. The TMLH-a gene with bacterially optimised codons was synthesised at GenScript. As a first step, the MBP-encoding gene MalE along … | Get A Quote |
Trimethyllysine hydroxylase (TMLH) catalyses the first step in carnitine biosynthesis - the conversion of N6,N6,N6-trimethyl-l-lysine to 3-hydroxy-N6,N6,N6-trimethyl-l-lysine. By changing carnitine availability it is possible to optimise cardiac energy metabolism, that is beneficial under certain ischemic conditions. Previous efforts have been devoted towards the inhibition of gamma-butyrobetaine dioxygenase, which catalyses the last step in carnitine biosynthesis. However, the effects of TMLH activity regulation are currently unexplored. To facilitate the development of specific ligands of TMLH, large quantities of recombinant protein are necessary for downstream binding and structural studies. Here, we descri... More
