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Efficient separation of α-ketoglutarate from Yarrowia lipolytica WSH-Z06 culture broth by converting pyruvate to l-tyrosine.

Bioresour Technol. 2019; 
Lei Q, Zeng W, Zhou J, Du G.
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Codon Optimization … The TPL gene from Citrobacter freundii (NCBI Accession No. L10821.1) was codon-optimized and synthesized by GenScript Corporation (Nanjing, China), and cloned into the pET-28a(+) vector with restriction sites BamHI/HindIII … Get A Quote

摘要

Co-production of α-ketoglutaric acid (KGA) and pyruvic acid (PYR) by Yarrowia lipolytica WSH-Z06 could significantly increase the final titer and yield of keto acids. However, efficient separation of KGA and PYR in an economic manner is a big challenge owing to their similar properties. In the present study, a separation process was established to convert PYR in the fermentation broth to l-tyrosine (TYR). Owing to its low solubility, TYR was easily precipitated out and could be easily removed from the reaction system. The whole-cell catalysis reaction solution was subjected to acid treatment, centrifugation, cation exchange column separation, rotary evaporation, Buchner funnel filtration, and dry separation me... More

关键词

Co-production; Extraction; Keto acids; Separation; Tyrosine phenol lyase