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Proteins, Expression, Isolation and Analysis | … The amino acidic sequences of KP-Cryst Wt and KP-Cryst Mut proteins are presented in Table 1. Nickel Magnetic Beads for His-Tag Protein Purification were available from GenScript, Molecular weight GRS Protein Marker Blue standards and culture medium were purchased … | Get A Quote |
Styling hair with straightening irons is a popular daily hair routine that significantly damage the hair keratin fiber due to the high temperature applied. In this study, we investigate the effect of two fusion proteins based on the human eye γD-crystallin conjugated with a keratin-based peptide (KP-Cryst Wt and KP-Cryst Mut) on hair exposed to thermal damage. The mutant form was designed to improve protein stability and promote interaction with the hair. Through the study, it was demonstrated the protection of Asian and Caucasian virgin hair's structure by the pretreatments with the KP-Cryst fusion proteins. After hair thermal exposure, a higher water content was quantified by TGA on the hair fibers pretreate... More