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Proteins, Expression, Isolation and Analysis | … Briefly, the antiserum was diluted in binding/wash buffer (0.15 m NaCl, 20 mm Na 2 HPO 4 , pH 8.0) and added to Protein A resin (GenScript, Nanjing, China) … The supernatant was pre-cleared with 30 μl of Protein A beads (GenScript) at 4 °C for 40 min with agitation … | Get A Quote |
Phagocytosis is a conserved cellular response among metazoans. Opsonins are some molecules that label targets to increase their susceptibility to phagocytosis. Opsonins are usually captured by receptors on the surface of phagocytes. Our previous study found the C-type lectin FcLec4 from Chinese white shrimp Fenneropenaeus chinensis might function as an opsonin to facilitate bacterial clearance. In the present study we purified the native FcLec4 protein and confirmed its opsonic activity in the near relation, kuruma shrimp Marsupenaeus japonicus. The possible receptor of FcLec4 was identified as β-integrin by panning a T7 phage display library of shrimp hemocytes and then confirmed by co-immunoprecipitation ass... More