| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | Synthesis of genes for medaka fshb (Accession Number NM_001309017.1), lhb(Accession Number AB541982.1), fshba, and lhba (gpa; Accession Number NM_001122906) was outsourced to GenScript, New Jersey, U.S.A. For each construct gene expression cassettes were generated with P. pastoriscodon optimized DNA sequence. | Get A Quote |
The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fshβ (mdFshβ), Lhβ (mdLhβ), Fshβα (mdFshβα), and Lhβα (mdLhβα) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fshβ and Lhβ by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFshβα and mdLhβα within enzyme-linked immunosorbent assays (ELISAs), in which protein amounts were analyzed related to body weight and age of th... More
