Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | A fragment of human AF9 (amino acids 406‒498) was expressed as a His-tag fusion protein in pET-16b, purified on NTA-agarose according to Qiagen’s protocol and by HPLC, and then sent to Genscript for immunization into rabbits. | Get A Quote |
X chromosome inactivation and genomic imprinting are two classic epigenetic regulatory processes that cause mono-allelic gene expression. In female mammals, mono-allelic expression of the long non-coding RNA gene X-inactive specific transcript (XIST) is essential for initiation of X chromosome inactivation upon differentiation. We have previously demonstrated that the central factor of super elongation complex-like 3 (SEC-L3), AFF3, is enriched at gamete differentially methylated regions (DMRs) of the imprinted loci and regulates the imprinted gene expression. Here, we found that AFF3 can also bind to the DMR downstream of the XIST promoter. Knockdown of AFF3 leads to de-repression of the inactive allele of XIS... More