Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | AZIN1 and ODC1 promoter regions containing putative AHR binding sites, as well as the ODC1 promoter including the MYC binding sites (either WT or mutated) were syn- thesized by Genscript and subcloned into the pGL3 promoter (AZI1 no. | Get A Quote |
Polyamine inhibition for cancer therapy is, conceptually, an attractive approach but has yet to meet success in the clinical setting. The aryl hydrocarbon receptor (AHR) is the central transcriptional regulator of the xenobiotic response. Our study revealed that AHR also positively regulates intracellular polyamine production via direct transcriptional activation of 2 genes, ODC1 and AZIN1, which are involved in polyamine biosynthesis and control, respectively. In patients with multiple myeloma (MM), AHR levels were inversely correlated with survival, suggesting that AHR inhibition may be beneficial for the treatment of this disease. We identified clofazimine (CLF), an FDA-approved anti-leprosy drug, as a poten... More