| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Cell adhesive peptides (GRGDSPC, GRDGSPC, CGGEGYGEGYIGSR, and CGGKAFDITYVRLKF; >95% purity; GenScript) were dissolved in 1× PBS containing 10 mM Hepes and added to PEG-4MAL solution to produce functionalized PEG- 4MAL precursors.... To synthesize cell-encapsulated hydrogels, the solution containing functionalized PEG-4MAL precursors and cells was mixed with protease-degradable cross-linking peptide (GCRDVPMSMRGGDRCG; GenScript) or non- degradable hexa(ethylene glycol) dithiol (Sigma-Aldrich) dissolved in 1× PBS containing 10 mM Hepes and subsequently polymerized at 37°C/5% CO2 for 5 min before adding MuSC growth media (F10 con- taining 1% penicillin/streptomycin, 1% GlutaMAX, and 20% horse serum). | Get A Quote |
Muscle satellite cells (MuSCs) play a central role in muscle regeneration, but their quantity and function decline with comorbidity of trauma, aging, and muscle diseases. Although transplantation of MuSCs in traumatically injured muscle in the comorbid context of aging or pathology is a strategy to boost muscle regeneration, an effective cell delivery strategy in these contexts has not been developed. We engineered a synthetic hydrogel-based matrix with optimal mechanical, cell-adhesive, and protease-degradable properties that promotes MuSC survival, proliferation, and differentiation. Furthermore, we establish a biomaterial-mediated cell delivery strategy for treating muscle trauma, where intramuscular injecti... More
