Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | The human UBA5 and UFM1 open read ing f rames were synthes ized (GenScript) and cloned into pET15b containing an N-terminal HisX6 Tag followed by a TEV protease–cleav- age site. | Get A Quote |
The modification of proteins by ubiquitin-fold modifier 1 (UFM1) is implicated in many human diseases. Prior to conjugation, UFM1 undergoes activation by its cognate activating enzyme, UBA5. UBA5 is a non-canonical E1 activating enzyme that possesses an adenylation domain but lacks a distinct cysteine domain. Binding of UBA5 to UFM1 is mediated via an amino acid sequence, known as the UFM1-interacting sequence (UIS), located outside the adenylation domain that is required for UFM1 activation. However, the precise boundaries of the UIS are yet not clear and are still under debate. Here we revisit the interaction of UFM1 with UBA5 by determining the crystal structure of UFM1 fused to 13 amino acids of human UBA5.... More