Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | injection of 300 µl emulsion containing 200 µg MOG35-55 (GenScript) in incomplete Freund’s adjuvant (ThermoFisherScientific) supplemented with killed Mycobacterium tuberculosis (BD; 2 mg/ml). | Get A Quote |
Genome-wide association studies as well as lymphatic expression analyses have linked both Cbl-b and GM-CSF to human multiple sclerosis as well as other autoimmune diseases. Both Cbl-b and GM-CSF have been shown to play a prominent role in the development of murine encephalomyelitis; however, no functional connection between the two has yet been established. In this study, we show that Cblb knockout mice demonstrated significantly exacerbated severity of experimental autoimmune encephalomyelitis (EAE), augmented T cell infiltration into the central nervous system (CNS) and strongly increased production of GM-CSF in T cells in vitro and in vivo.GM-CSF neutralization demonstrated that the increased susceptibility ... More