| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | Cloning and expression of recombinant proteins cDNA encoding the promiscuous T non-B cell epitope of TT (amino acid residues 830–844) followed by a di-lysine linker (KK) and mouse N-terminal Sp17 fragment corresponding to amino acid residues 1–80 (TT-KK-Sp17N)or C-terminal fragment of Sp17 corresponding to amino acid residues 76-126 (TT-KK-Sp17C) were PCR amplified from codon-optimized synthetic genes obtained from GenScript Corporation in pUC57 (Piscataway, NJ, USA) using appropriate forward and reverse primers (Table 6) containing NdeI and XhoI restriction sites. | Get A Quote |
Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino acid residues 76-126) terminal fragments of mouse sperm protein 17 (Sp17) fused to the promiscuous T non-B cell epitope of tetanus toxoid (TT), amino acid residues 830-844 followed by di-lysine linker (KK) (TT-KK-Sp17N or TT-KK-Sp17C , respectively) or (ii) mouse equatorin (amino acid residues 21-185) fused to the T non-B cell epitope of bovine RNase (amino acid residues 94-104) were expressed in Escherichia coli. Immunization of female FVB/J mice, using alum as an adjuvant, led to the generation of high antibody ti... More
