Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | All Fmoc- and side chain-protected amino acids, low-loading Fmoc-Gly-Wang resin and other components for solid-phase pep- tide synthesis were purchased from EMD Millipore except for HATU and HOAt, which were obtained from GenScript. | Get A Quote |
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are an emerging class of natural products that harbor diverse chemical functionalities, usually introduced via the action of a small number of tailoring enzymes. We have been interested in RiPP biosynthetic gene clusters that encode unusual metalloenzymes, as these may install as yet unknown alterations. Using a new bioinformatic search strategy, we recently identified an array of unexplored RiPP gene clusters that are quorum sensing-regulated and contain one or more uncharacterized radical S-adenosylmethionine (RaS) metalloenzymes. Herein, we investigate the reaction of one of these RaS enzymes and find that it installs an intramolecula... More