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TYW1: A Radical SAM Enzyme Involved in the Biosynthesis of Wybutosine Bases.

Methods Enzymol. 2018;

Young AP, Bandarian V.

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Codon Optimization	jannaschii was obtained in codon-optimized form (GenScript) (see Fig.... coli, was synthesized (GenScript) and cloned between the NdeI and HindIII sites of pET29a vector to create pAY423 (see Fig.	Get A Quote

摘要

Transfer RNA is extensively modified by the actions of a variety of enzymes. The radical S-adenosyl-l-methionine enzyme TYW1 modifies tRNAPhe forming the characteristic tricyclic ring via the condensation of carbons 2 and 3 of pyruvate. This chapter details methods that are required for studies of TYW1.,© 2018 Elsevier Inc. All rights reserved.