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Whole-Genome Phage Display Libraries: A Powerful Tool for Antigen Discovery.

Methods Mol Biol. 2019; 
Beghetto E, Gargano N.
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Catalog Antibody The antibodies are not commercially available and should be made using custom services provided by antibody developers (e.g., Pierce, GenScript, etc.) using PEG-purified λKM4 bacteriophages as the antigen. Get A Quote

摘要

In the last two decades, phage display technology has been used for investigating complex biological processes and isolating molecules of practical value in several applications. Bacteriophage lambda, representing a classical cloning and expression system, has also been exploited for generating display libraries of small peptides and protein domains. More recently, large cDNA and whole-genome lambda display libraries of human pathogens have been generated for the discovery of new antigens for biomedical applications. Here, we describe the construction of a whole-genome library of a common pathogen-Streptococcus pneumoniae-and the use of this library for the molecular dissection of the human B-cell response agai... More

关键词

Antigen discovery; Bacteriophage lambda; Genomic DNA display; Phage display library; Streptococcus pneumoniae