Products/Services Used | Details | Operation |
---|---|---|
Proteins, Expression, Isolation and Analysis | The protein was purified by High-Affinity Ni-NTA Resin (GenScript Inc...., Nanjing, China) or Glutathi- one Resin (GenScript Inc. | Get A Quote |
The functional significance of palmitoylation in the switch between self-renewal and differentiation of neural stem cells (NSCs) is not well defined, and the underlying mechanisms of protein palmitoylation are not well understood. Here, mouse NSCs were used as a model system and cell behavior was monitored in the presence of the protein palmitoylation inhibitor 2-bromopalmitate (2BRO). Our data show that 2BRO impaired the differentiation of NSCs into both neurons and glia and impaired NSC cell cycle exit. Moreover, the results show that palmitoylation modified E1A-like inhibitor of differentiation one (EID1) and this modification regulated EID1 degradation and CREB-binding protein (CBP)/p300 histone acetyltrans... More