Products/Services Used | Details | Operation |
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Catalog Antibody | For immunocomplex kinase assay, protein extract (100 mg) was incubated with anti- OsDMI3 antibody (2 mg; Genscript Biotechnology, Nanjing, China) or anti-OsMPK1 antibody (2 mg; Genscript Biotech- nology) in an immunoprecipitation buffer as described pre- viously (Zhang et al. | Get A Quote |
In rice, the Ca(2+) /calmodulin (CaM)-dependent protein kinase (CCaMK) OsDMI3 has been shown to be required for abscisic acid (ABA)-induced antioxidant defence. However, it is not clear how OsDMI3 participates in this process in rice. In this study, the cross-talk between OsDMI3 and the major ABA-activated MAPK OsMPK1 in ABA-induced antioxidant defence was investigated. ABA treatment induced the expression of OsDMI3 and OsMPK1 and the activities of OsDMI3 and OsMPK1 in rice leaves. In the mutant of OsDMI3, the ABA-induced increases in the expression and the activity of OsMPK1 were substantially reduced. But in the mutant of OsMPK1, the ABA-induced increases in the expression and the activity of OsDMI3 were not ... More