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  Validation of a yeast malate dehydrogenase 2 (Mdh2) antibody tested for use in western blots

F1000Research. 2018-07; 
Shiran Gabay-Maskit, Maya Schuldiner, Einat Zalckvar
Products/Services Used Details Operation
Catalog Antibody In order to identify specifically the Mdh2 isozyme, GenScript USA produced three different antibodies that we further tested by western blot.... cerevisae Mdh2 were custom produced for us by GenScript USA : 1. Get A Quote

摘要

Malate dehydrogenases (Mdhs) reversibly convert malate to oxaloacetate and serve as important enzymes in several metabolic pathways. In the yeast  Saccharomyces cerevisiae there are three Mdh isozymes, localized to different compartments in the cell. In order to identify specifically the Mdh2 isozyme, GenScript USA produced three different antibodies that we further tested by western blot. All three antibodies recognized the S. cerevisiae Mdh2 with different background and specificity properties. One of the antibodies had a relatively low background and high specificity and thus can be used for specific identification of Mdh2 in ... More

关键词

Malate dehydrogenase, Saccharomyces cerevisiae, antibody, western blot