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Proteins, Expression, Isolation and Analysis | … were stained with Hoechst 33342, a blue-fluorescence dye to stain all cells and propidium iodide (PI, Sigma Aldrich, Dorset, UK), a red-fluorescence dye to stain dead cells, according to double Hoechst 33342/PI stain apoptosis detection kit (GenScript, Leiden, Netherlands) … | Get A Quote |
Tumour-promoting inflammation is involved in colorectal cancer (CRC) development and therapeutic resistance. However, the antibiotics and antibacterial drugs and signalling that regulate the potency of anticancer treatment upon forced differentiation of cancer stem-like cell (CSC) are not fully defined yet. We screened an NIH-clinical collection of the small-molecule compound library of antibacterial/anti-inflammatory agents that identified potential candidate drugs targeting CRC-SC for differentiation. Selected compounds were validated in both in vitro organoids and ex vivo colon explant models for their differentiation induction, impediment on neoplastic cell growth, and to elucidate the mechanism of their an... More