| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Cells were cultured at 37˚C in DMEM supplemented with 10% (v/v) FCS, 2-ME (50 mM), ʟ-glutamine (2 mM), penicillin (100 U/ml), and streptomycin (100 mg/ml) for 5 h total in the presence or absence of 5 mM gD315–327 peptide (GenScript). | Get A Quote |
CD4 T cell responses are crucial for the control of many intracellular pathogens， yet the requirements for their induction are not fully understood. To better understand the role that various dendritic cell (DC) subtypes play in CD4 T cell priming， we compared in vivo T cell responses to skin inoculation of mice with infectious or UV-inactivated HSV type 1. Localized infection elicited a Th1 response that was primed in skin-draining lymph nodes involving Ag presentation by migratory dermal and lymph node-resident DC. However， expansion and Th1 differentiation was impaired in response to UV-inactivated virus (UV-HSV)， and this defect correlated with a restriction of Ag presentation to migratory CD103 der... More
