| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | The cultures were inoculated (1%, v/v) into MRS broth without antibiotics and grown at 37 °C until OD600nm reached ∼0.30, then supplemented with 100 ng/mL sakacin P (SppIP) (Genscript). | Get A Quote |
Genome engineering is essential for application of synthetic biology in probiotics including lactobacilli and bifidobacteria. Several homologous recombination system-based mutagenesis tools have been developed for these bacteria， but still have many limitations in different species or strains. Here we developed a genome engineering method based on an inducible self-destruction plasmid delivering homologous DNA into bacteria. Excision of the replicon by induced recombinase facilitates selection of homologous recombination events. This new genome editing tool called inducible plasmid self-destruction (IPSD) was successfully used to perform gene knockout and knock-in in lactobacilli and bifidobacteria. Due to it... More
