Products/Services Used | Details | Operation |
---|---|---|
Proteins, Expression, Isolation and Analysis | … Insert cassettes were 410 synthesized by <b>Genscript</b> containing BssHII and ApaI restriction sites<br> at the 5' and 3' 411 termini, respectively … Inserts containing miR-target 415 and mismatch<br> sequences were digested from <b>Genscript</b> constructs using BssHII and 416 Page 21. 21 … | Get A Quote |
Chikungunya virus (CHIKV) is an arbovirus capable of causing a severe and often debilitating rheumatic syndrome in humans. CHIKV replicates in a wide variety of cell types in mammals, which has made attributing pathologic outcomes to replication at specific sites difficult. To assess the contribution of CHIKV replication in skeletal muscle cells to pathogenesis, we engineered a CHIKV strain exhibiting restricted replication in these cells via incorporation of target sequences for skeletal muscle cell-specific miR-206. This virus, which we term SKE, displayed diminished replication in skeletal muscle cells in a mouse model of CHIKV disease. Mice infected with SKE developed less severe disease signs, including di... More