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Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.

Nat. Chem. Biol.. 2018;

Liszczak Glen，Diehl Katharine L，Dann Geoffrey P，Muir T

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Proteins, Expression, Isolation and Analysis	Next, 20 μ L of pre-equilibrated (in binding buffer) Flag-agarose affinity resin (G1 affinity resin; Genscript) was added to the binding reaction and incubated on an end-over-end rotator at 4 °C for an additional 90 min....com/naturechemicalbiology of olaparib) and added to 100 μ L of pre-equilibrated Flag-agarose affinity resin (G1 affinity resin; Genscript).	Get A Quote

摘要

Recent studies report serine ADP-ribosylation on nucleosomes during the DNA damage response. We unveil histone H3 serine 10 as the primary acceptor residue for chromatin ADP-ribosylation and find that specific histone acetylation marks block this activity. Our results provide a molecular explanation for the well-documented phenomenon of rapid deacetylation at DNA damage sites and support the combinatorial application of PARP and HDAC inhibitors for the treatment of PARP-dependent cancers.