Products/Services Used | Details | Operation |
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Catalog Antibody | The following antibodies were used: anti-Band3 antibody (ab108414, Abcam), anti-pan cadherin antibody (RB-9036- PU, Neomarkers Inc, Fremont CA), anti-mouse apoM antibody (A00954, GenScript Co, Pis- kataway, NJ), anti-apoA-I antibody (AB740, Chemicon International Inc. | Get A Quote |
Sphingosine 1-phosphate (S1P) is a bioactive lipid mediator that is thought to be involved in various diseases. Although the main source of S1P in the plasma is erythrocytes, how S1P is exported from erythrocytes has not been elucidated. When we differentiated K562 cells into erythroblast-like cells with sodium butyrate, we observed that the efflux of S1P was increased without increased expression of previously proposed S1P transporters, while the expression levels of Band3 were increased. Therefore, in this study, we investigated the involvement of Band 3, the most characteristic membranous transporter for erythrocytes, in S1P efflux, using 4,4'-diisothiocyanatodihydrostilbene-2,2'-disulfon... More