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Proteins, Expression, Isolation and Analysis | Endotoxin removal from protein solution was achieved primarily by 10kD ultra- filtration membrane (Milipore), further followed by polymyxin B affinity column in ToxinEraserTM Endotoxin Removal Kit (GenScript).... Endotoxin content was semi-quantified via ToxinSensorTM Gel Clot Endotoxin Assay Kit (GenScript). | Get A Quote |
Alzheimer's disease (AD) is one of the leading causes of death for people over 65 years. Worse still, no completely effective therapeutic agent is available so far. One important pathological hallmark of AD is accumulated amyloid-β (Aβ) plaques with dysregulated metal homeostasis. Human metallothionin 3 (MT3), a regulator of metal homeostasis, is downregulated at least 30% in AD brain. So far, some in vitro studies demonstrated its multiple functions related to AD. However, it is a great pity that systematic in vivo studies of MT3 on AD model animals are still a blank so far. In this study, we treated APP/PS1 mice with sustained drug release of ZnMT3 directly to the central nervous system, and i... More