Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | 00-4506-51 CFSE Sigma CAS Number: 150347-59-4 M57, SCLEFWQRV; Genscript Custom m139, TVYGFCLL; Genscript Custom SIINFEKL - N4 Genscript Custom Poly I:C Sigma-Aldrich CAS Number: 42424-50-0 Glucose Sigma-Aldrich CAS Number: 50-99-7 2-[1-14C] deoxyglucose PerkinElmer NEC495A050UC Insulin (fast working insulin) Novo Nordisk Insulin (basal) Novo Nordisk Pyruvate sodium solution Sigma-Aldrich Cas Number: 113-24-6 CCL4 Sigma-Aldrich CAS Number: 56-23-5 Mineral oil Sigma-Aldrich CAS Number: 8042-47-5 Paracetamol Sigma-Aldrich CAS Number: 103-90-2 MCC950 Cayman Chemical CAS Number 210826-40-7 Diphteria Toxin Merck Cat. | Get A Quote |
Pro-inflammatory cytokines of a T helper-1-signature are known to promote insulin resistance (IR) in obesity, but the physiological role of this mechanism is unclear. It is also unknown whether and how viral infection induces loss of glycemic control in subjects at risk for developing diabetes mellitus type 2 (DM2). We have found in mice and humans that viral infection caused short-term systemic IR. Virally-induced interferon-γ (IFN-γ) directly targeted skeletal muscle to downregulate the insulin receptor but did not cause loss of glycemic control because of a compensatory increase of insulin production. Hyperinsulinemia enhanced antiviral immunity through direct stimulation of CD8 effector T cell function... More