Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | Blots were developed by the chemiluminescent method using the LumiSensor 688 Chemiluminescent HRP Substrate kit (Genscript USA, Lab Supplies Scientific SA, Hellas) 689 and SuperRX Fuji medical X-Ray films (FujiFILM Europe, Lab Supplies Scientific SA, 690 Hellas). | Get A Quote |
The AzgA purine/H(+) symporter of Aspergillus nidulans is the founding member of a functionally and phylogenetically distinct transporter family present in fungi, bacteria and plants. Here a valid AzgA topological model is built based on the crystal structure of the Escherichia coli uracil transporter UraA, a member of the nucleobase-ascorbate transporter (NAT/NCS2) family. The model consists of 14 transmembrane, mostly α-helical, segments (TMSs) and cytoplasmic N- and C-tails. A distinct compact core of 8 TMSs, made of two intertwined inverted repeats (TMSs 1-4 and 8-11), is topologically distinct from a flexible domain (TMSs 5-7 and 12-14). A putative substrate binding cavity is visible between t... More