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Catalog Antibody | The proteins were electrophoretically trans- ferred to a nitrocel lu lose membrane and detected with either anti-DVSSJ1 mouse Ab (1:2,500, Genscr ipt, USA) or anti-DVSSJ2 hybridoma supernatant mouse Ab (1:50, Genscript, USA), followed by goat anti-mouse (GAM)-HRP conjugated secondary Ab (Bio-Rad, Hercules, CA) (1: 12,500). | Get A Quote |
RNA interference (RNAi) is a promising new technology for corn rootworm control. This paper presents the discovery of new gene targets - dvssj1 and dvssj2, in western corn rootworm (WCR). Dvssj1 and dvssj2 are orthologs of the Drosophila genes snakeskin (ssk) and mesh, respectively. These genes encode membrane proteins associated with smooth septate junctions (SSJ) which are required for intestinal barrier function. Based on bioinformatics analysis, dvssj1 appears to be an arthropod-specific gene. Diet based insect feeding assays using double-stranded RNA (dsRNA) targeting dvssj1 and dvssj2 demonstrate targeted mRNA suppression, larval growth inhibition, and mortality. In RNAi treated WCR, injury to... More