| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | In vitro MDSC immunosuppressive function To measure the immunosuppressive function of MDSCs transduced with Olfr29-ps1 shRNA or Olfr29-ps1/lentiviruses, the splenocytes obtained from OT-I or OT-II mice were cocultured with MDSCs in presence of 200 nM OVA peptide (OVA257-264, GenScript) or OVA peptide (OVA323-339, GenScript) in 96-well plates at a ratio of 1:0, 1:1, 1:1/2, 1:1/4, 1:1/8, and 0:1 for 48 hours. | Get A Quote |
Long noncoding RNA (lncRNA) plays a critical role in many biological processes， such as cell differentiation and development. However， few studies about lncRNAs regulating the differentiation and development of myeloid-derived suppressor cells (MDSCs) exist. In this study， we identified a lncRNA pseudogene， ， which was expressed in MDSCs and upregulated by the proinflammatory cytokine IL6. The in vertebrates is conserved， and the similarity between the and human sequence is 43%. This lncRNA promoted the immunosuppressive function and differentiation of monocytic (Mo-)MDSCs and It directly sponged to downregulate miR-214-3p， which may target MyD88 to modulate the differentiation and developme... More
