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Proteins, Expression, Isolation and Analysis | 20 μg of extracted protein from transfected and control cells were run and se- parated on an ExpressPlusTM PAGE Gel 8% (GenScript, Piscataway, USA) and transferred to polyvinylidene difluoride (PVDF, Thermo Fisher) membranes. | Get A Quote |
Hemoglobinopathies, such as β-thalassemia, and sickle cell disease (SCD) are caused by abnormal structure or reduced production of β-chains and affect millions of people worldwide. Hereditary persistence of fetal hemoglobin (HPFH) is a condition which is naturally occurring and characterized by a considerable elevation of fetal hemoglobin (HbF) in adult red blood cells. Individuals with compound heterozygous β-thalassemia or SCD and HPFH have milder clinical symptoms. So, HbF reactivation has long been sought as an approach to mitigate the clinical symptoms of β-thalassemia and SCD. Using CRISPR-Cas9 genome-editing strategy, we deleted a 200bp genomic region within the human erythroid-specific BCL11... More