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Proteins, Expression, Isolation and Analysis | 6 Western blot Equal amounts of proteins extracted from cultured GCs using radio immune p recipitat ion assay buffer (Beyot ime Inst itute of Biotechnology, Shanghai, China) were fract ioned by electrophoresis on a 4-20 % Sure PAGE gel (Genscript, Nanjing, China) and transferred to PVDF membranes (Millipore, Bedford, MA) by electroblotting. | Get A Quote |
As the follicle develops, the thickening of the granulosa compartment leads to progressively deficient supply of oxygen in GCs due to the growing distances from the follicular vessels. These conditions are believed to cause hypoxia in GCs during folliculogenesis. Upon hypoxic conditions, several types of mammalian cells have been reported to undergo cell cycle arrest. However, it remains unclear whether hypoxia exerts any impact on cell cycle progression of GCs. On the other hand, although the GCs may live in a hypoxic environment, their mitotic capability appears to be unaffected in growing follicles. It thus raises the question whether there are certain intra-ovarian factors that might overcome the ... More