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A nemertean excitatory peptide/CCHamide regulates ciliary swimming in the larvae of .

Front. Zool.. 2019;

ThielDaniel，BauknechtPhilipp，JékelyGáspár，HejnolAnd

Products/Services Used	Details	Operation
Peptide Synthesis	longissimus EP1 and EP2 (customized from GenScript; sequences as described below) were diluted to different concentrations , the dose-dependent luminescence re- sponse was measured in a plate reader (BioTek Synergy Mx and Synergy H4, BioTek , Winooski, USA) and ana- lyzed with Prism 6 (GraphPad, La Jolla , USA).... Immunohistochemistry Antibodies against the C-terminal CAAGNGamide se- quence of the Lineus excitatory peptide were raised in rab- bits (genscript) and the antibodies were purified from the blood serum with a Sulfolink™ Immobilization Kit (Ther- moFisher Scientific) according to [48].	Get A Quote

摘要

The trochozoan excitatory peptide (EP) and its ortholog， the arthropod CCHamide， are neuropeptides that are only investigated in very few animal species. Previous studies on different trochozoan species focused on their physiological effect in adult specimens， demonstrating a myo-excitatory effect， often on tissues of the digestive system. The function of EP in the planktonic larvae of trochozoans has not yet been studied.