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Proteins, Expression, Isolation and Analysis | Next, 80 µg of protein were separated on 10% SDS-PAGE gels (GenScript, Piscataway, NJ, USA) and electrotransferred to polyvinylidene difuoride (PVDF) membranes (Bio-Rad Laboratories Inc., Hercules, CA, USA, cat.# 1704157). | Get A Quote |
Pannexins (Panxs), glycoproteins that oligomerize to form hemichannels on the cell membrane, are topologically similar to connexins, but do not form cell-to-cell gap junction channels. There are 3 members of the family, 1-3, with Panx1 being the most abundant. All Panxs are expressed in bone, but their role in bone cell biology is not completely understood. We now report that osteocytic Panx1 deletion (Panx1) alters bone mass and strength in female mice. Bone mineral density after reaching skeletal maturity is higher in female Panx1 mice than in control Panx1 mice. Further, osteocytic Panx1 deletion partially prevented aging effects on cortical bone structure and mechanical properties. Young 4-mon... More