Products/Services Used | Details | Operation |
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Catalog Antibody | Membranes were incubated with primary antibodies against postsynaptic proteins PSD-95 (Neuromab, 1:20.000), GluN1 (Neuromab, 1:1.000), GluN2A (Epitomics, 1:5.000), GluA1 (Abcam, 1:50.000), GluA2 (Neuromab, 1:2.000), mGluR2 (Abcam, 1:2.000), and presynaptic proteins Munc18 (1:1000, gift from M. Verhage, VU University Amsterdam, The Netherlands), synaptotagmin (Hybrodoma, 1:1.000), synaptophysin (Genscript, 1:1.000), syntaxin (Genscript, 1:500), synaptobrevin (SySy, 1:5.000), and SNAP-25 (Sternberger, 1:10.000). | Get A Quote |
The brain is considered to be autonomous in lipid synthesis with astrocytes producing lipids far more efficiently than neurons. Accordingly, it is generally assumed that astrocyte-derived lipids are taken up by neurons to support synapse formation and function. Initial confirmation of this assumption has been obtained in cell cultures, but whether astrocyte-derived lipids support synapses in vivo is not known. Here, we address this issue and determined the role of astrocyte lipid metabolism in hippocampal synapse formation and function in vivo. Hippocampal protein expression for the sterol regulatory element-binding protein (SREBP) and its target gene fatty acid synthase (Fasn) was found in astrocytes but... More