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Proteins, Expression, Isolation and Analysis | For protein analysis of bacteriocin-enriched fractions 300 µL aliquots were ultracentrifuged at Rmax of 287,582 x g at 4°C for 15 minutes, pellets were resuspended in 40 µL of Laemmli buffer and 3-6 µL were loaded on a 8-16% PAGE gel (Genscript). | Get A Quote |
R-type bacteriocins are contractile phage tail-like structures that are bactericidal towards related bacterial species. The C-terminal region of the phage tail fiber protein determines target-binding specificity. The mutualistic bacteria Xenorhabdus nematophila and X. bovienii produce R-type bacteriocins (xenorhabdicins) that are selectively active against different Xenorhabdus species. We analyzed the P2-type remnant prophage clusters in draft sequences of nine strains of X. bovienii The C-terminal tail fiber region in each of the respective strains was unique and consisted of mosaics of modular units. The region between the main tail fiber gene (xbpH1) and the sheath gene (xbpS1) contained a variable number o... More