Products/Services Used | Details | Operation |
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Catalog Antibody | Sections were blocked with 3% H2O2 for 10 min., blocked in 6% Bovine Serum Albumin (BSA, Sigma) for 15-min and incubated with the custom primary antibodies: 6 anti-RSPO1 (GenScript, 674196-52), anti-WNT4 (GenScript, 674196-25), anti-β-catenin (GenScript, 674196-35), and anti-SOX9a (GenScript, 674196-42) at RT. | Get A Quote |
Development of the gonads is a complex process, which starts with a period of undifferentiated, bipotential gonads. During this period the expression of sex-determining genes is initiated. Sex determination is a process triggering differentiation of the gonads into the testis or ovary. Sex determination period is followed by sexual differentiation, i.e. appearance of the first testis- and ovary-specific features. In Xenopus laevis W-linked DM-domain gene (DM-W) had been described as a master determinant of the gonadal female sex. However, the data on the expression and function of other genes participating in gonad development in X. laevis, and in anurans, in general, are very limited. We applied ... More