Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | . Purified IgM (Rockland, 003-0107) and IgY (GenScript, A01010) were used to quantitate plasma Ig titers | Get A Quote |
Transgenic animal platforms for the discovery of human monoclonal antibodies have been developed in mice, rats, rabbits and cows. The immune response to human proteins is limited in these animals by their tolerance to mammalian-conserved epitopes. To expand the range of epitopes that are accessible, we have chosen an animal host that is less phylogenetically related to humans. Specifically, we generated transgenic chickens expressing antibodies from immunoglobulin heavy and light chain loci containing human variable regions and chicken constant regions. From these birds, paired human light and heavy chain variable regions are recovered and cloned as fully human recombinant antibodies. The human antibo... More