NUCKS is a vertebrate specific， nuclear and DNA-binding phospho protein. The protein is highly expressed in rapidly dividing cells， and is overexpressed in a number of cancer tissues. The phosphorylation of NUCKS is cell cycle and DNA-damage regulated， but little is known about the responsible kinases. By utilizing in vitro and in vivo phosphorylation assays using isolated NUCKS as well as synthetic NUCKS-derived peptides in combination with mass spectrometry， phosphopeptide mapping， phosphphoamino acid analyses， phosphospecific antibodies and the use of specific kinase inhibitors， we found that NUCKS is phosphorylated on 11 sites by CK2. At least 7 of the CK2 sites are phosphorylated in vivo. We ... More
NUCKS is a vertebrate specific， nuclear and DNA-binding phospho protein. The protein is highly expressed in rapidly dividing cells， and is overexpressed in a number of cancer tissues. The phosphorylation of NUCKS is cell cycle and DNA-damage regulated， but little is known about the responsible kinases. By utilizing in vitro and in vivo phosphorylation assays using isolated NUCKS as well as synthetic NUCKS-derived peptides in combination with mass spectrometry， phosphopeptide mapping， phosphphoamino acid analyses， phosphospecific antibodies and the use of specific kinase inhibitors， we found that NUCKS is phosphorylated on 11 sites by CK2. At least 7 of the CK2 sites are phosphorylated in vivo. We also found that NUCKS is phosphorylated on two sites by ATM kinase and DNA-PK in vitro， and is phosphorylated in vivo by ATM kinase in γ-irradiated cells. All together， we identified three kinases phosphorylating 13 out of 39 in vivo phosphorylated sites in mammalian NUCKS. The identification of CK2 and PIKK kinases as kinases phosphorylating NUCKS in vivo provide further evidence for the involvement of NUCKS in cell cycle control and DNA repair.
